Lab Nanotechnology Group
The personal researcher carries out his daily work in two main areas of work. The first one, a laboratory of around 30 m2, is principally oriented to organic synthesis. It is equipped with two fume hoods, a large bench, a rotary evaporator, a refrigerated centrifuge, an evaporating centrifuge, a thermo-block, two gel electrophoresis chambers, two power supplies, two balances, a refrigerator, a freezer, diverse glassware and plasticware. The laboratory also have a MerMade4 DNA synthesizer to prepare oligonucleotides and “The Mermades” (Picture 1). The second laboratory is oriented to biological applications. This one is also equipped with a large bench, a balance, a refrigerator, a freezer, a vortex, a centrifuge, diverse glassware and plasticware. Moreover, an Akta prime chromatographer with columns for protein purification is located in the cool room (Picture 2). On the same floor, there is the cell culture facility equipped with the standard equipment for cell culture experiments: two cell culture hoods, two CO2 incubators, microscope, centrifuge, water bath, refrigerators, and freezers. In addition, there are assorted equipment in the institution we can use such as, fluorescent microscope, UV-Vis spectrophotometers (UV-Vis-NIR Cary 5000, Espectrofotómetro UV-Vis Varian), Circular Dichroism spectrophotometer J-815 , fluorimeter Fluoromax-4 Horiba, Microcalorimeter VP-ITC, thermogravimetric analyser TGA Q500, thermocycler, gel documentation system, centrifuge, ultra-low freezer (-80 °C), refrigerated orbital incubator shackers and laminar flow hood (for bacteria use only), among others (Pictures 3 and 4). Furthermore, the institution holds a Good Practice Laboratory for the characterization of Nanomaterials, with a Dynamic Light Scattering instrument Malvern Zetasizer Nano ZS, a pHmeter, a microplate reader Lector Biotek and micro-balance (0.01) (Picture 5 and 6). Also, the PIs are members of an associated unit between the Spanish National Center for Biotechnology (CNB-CSIC) and IMDEA Nanociencia, and through that arrangement our group has access to many facilities such as electron microscopy, confocal microscopy, proteomics, genomics, flow cytometry, animal facility, histology, and tissue culture among others. The CNB is on the same campus within walking distance from IMDEA Nanociencia. Finally, within the campus, there are additional institutions, such as Molecular Biology Center, Universidad Autónoma de Madrid, Materials Science Institute, Scientific Park, with complementary equipment or facilities.