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Dr. Cristina FLORS

cristina flors

Following my degree in Chemistry, I completed my PhD at the Institut Químic de Sarrià in Barcelona in 2004 under the supervision of Prof. Santi Nonell. During that time, I studied the photophysical properties of phenalenone derivatives, with particular emphasis on singlet oxygen photosensitization, using a range of spectroscopic techniques. In 2005 I moved to the laboratory of Prof. Johan Hofkens at the Katholieke Universiteit Leuven, Belgium, to learn single-molecule and super-resolution fluorescence microscopy. I investigated the photophysical properties of different molecules such as perylene diimide dendrimers and a range of fluorescent proteins. My most representative result from that period was the single-molecule characterization of the photoswitching properties of the fluorescent protein Dronpa and its mutants. Importantly, we showed how the thorough understanding of photophysics can help optimize super-resolution imaging (Flors et al, J. Am Chem. Soc. 2007). Having gained expertise in a new technique with great potential, I moved to the University of Edinburgh in 2008 to begin my independent research career, funded by the Engineering and Physical Sciences Research Council and The Royal Society. I started a new research program to develop methodology for super-resolution imaging of DNA based on single-molecule localization (Flors et al, ChemPhysChem 2009; Curr. Op. Chem. Biol. 2011). In February 2012 I moved to IMDEA Nanoscience with a Ramón y Cajal fellowship, and I am now Senior Group Leader. At IMDEA I continue working on the improvement of super-resolution fluorescence microscopy methods, most recently combining them with atomic force microscopy. In parallel to the super-resolution work, I am also interested in the photosensitizing properties of fluorescent proteins and their applications in advanced microscopy and phototherapy.

Phone: +34 912998767

Position: Senior Research Prof.

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alberto rpulido

I received my PhD in Chemistry from the Complutense University of Madrid, Spain, in 2009. In the group of Prof. Emilio Aicart & Prof. Elena Junquera, I investigated biocompatible DNA-carriers (-liposomes and -micelles) which could be explotaible for gene therapy. In 2010 I moved to the Zernike Institute for Advanced Materials at the University of Groningen, the Netherlands. In the group of Prof. Andreas Herrmann, I improved my expertise in imaging and biophysical techniques, oligonucleotide- and peptide-functionalization and payload delivery. My most successful achievement consisted in developing DNA-block copolymer-functionalized nanobiocontainers which are sequence-specifically programmed to release cargo by hybridization with complementary DNA-photosensitizer conjugates.
Looking for industrial applicability to my research, during 2013 I had also the chance to manage, design and implement a R&D project based on functionalized nanovehicles passing through the stratum corneum, at a cosmetic startup company. After that, I was awarded with an Experienced Research AMAROUT-II fellow to join IMDEA-Nanoscience Institute. Since 2014, I am working with Cristina Flors, investigating more efficient genetically-encoded singlet oxygen photosensitizers in order to improve photodynamic therapy and electron microscopy.

Phone: +34 912998898

Position: Postdoctoral Researcher

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Patricia BONDIA

patricia bondia

The development of new technologies to study the assembly of nanoscale events that rule every biological process has become my motivation for doing research since I studied Biotechnology (Polytechnic University of Valencia and Ghent University, 2013). During that time, I had the opportunity to work at the molecular biology lab at the IBMCP (Valencia), where my interest in the control of protein expression and genetic engineering began. I was fascinated by the applications of molecular biology in Biomedicine, and I decided to join the pharma industry becoming part of the Protein Science group of Crucell B.V. (Leiden, The Netherlands). There I discovered the power of biophysics to understand the protein nanoworld. Afterwards, I decided to enrol in the Biophysics master (Autonomous University of Madrid, 2014) and focus my master thesis on studying the role of proteins as a mechanical signal transductor through force spectroscopy at the Cajal Institute (Madrid). During this master, I was given the chance to discover state-of-art technology used in molecular biology research, and the new high resolution imaging techniques like super-resolution fluorescence microscopy captured my attention.

As a result, I am now doing my PhD in the group of Cristina Flors. My studies are focused on the application of super-resolution fluorescence microscopy in correlation with AFM for the nanoscale study of biological structures with potential application in biomedicine and materials science.

Phone: +34 912998765

Position: PhD Student 

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adrian valle

I started my academic training at the Polytechnic University of Madrid (UPM, 2014), where I studied Technical Industrial Engineering, with a specialization in Industrial Chemistry. During this stage, I was able to build a solid engineering base and begin to deepen my knowledge in advanced chemistry. I developed a heat pump installation to improve energy efficiency in a continuous distillation system, which allowed me to win the Gas Natural Fenosa award to the best final degree project in the chemical engineering area. I then enrolled at the Chemical Engineering Master at the Autonomous University of Madrid (UAM, 2016). For my master thesis I developed ceramic scaffolds for bone regeneration at the Institute of Ceramics and Glass in Madrid, and spent some time at the Belgian Ceramic Research Center in Mons, where I used the freeze-casting technology. I found this experience very fascinating, so I decided to immerse myself into research. Currently, I work in IMDEA Nanoscience, doing my PhD in the group of Cristina Flors. My objectives are to extend the potential of correlative super-resolution fluorescence microscopy and atomic force microscopy, and use this tool in microbiology.

Phone: +34 912998748

Position: PhD Student 

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